Binding of lactoferrin and free secretory component to enterotoxigenic Escherichia coli.
نویسندگان
چکیده
The ability of two glycoproteins of human milk, lactoferrin and free secretory component, to bind to Escherichia coli colonization factors (CFAs) was investigated using immunocytochemistry assays of enriched fimbrial extracts. The results revealed that lactoferrin binds to fimbrial CFA I adhesin but not to CFA II adhesin (CS1 and CS3), while free secretory component interacts with both CFA I and CFA II adhesins. Our data indicate that lactoferrin and free secretory component, which are very abundant proteins of human milk, could play an important role against infant enteric disease by blocking bacterial adhesion.
منابع مشابه
Free secretory component and lactoferrin of human milk inhibit the adhesion of enterotoxigenic Escherichia coli.
The non-immunoglobulin component of human milk responsible for the inhibition of Escherichia coli cell adhesion (haemagglutination) mediated by colonisation factor antigen 1 (CFA1) was determined by chromatographic fractionation of human whey proteins with Sephadex G-200, DEAE cellulose and heparin-sepharose. Pure free secretory component (fSC) and pure lactoferrin (Lf) were isolated and both c...
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ورودعنوان ژورنال:
- FEMS microbiology letters
دوره 203 1 شماره
صفحات -
تاریخ انتشار 2001